![]() SnapGene Viewer is software that allows you to create, browse, and share richly annotated DNA sequence files. Each time you edit a sequence or simulate cloning or PCR or mutagenesis, the procedure is automatically logged in a graphical history. SnapGene automatically records the steps in a cloning project. It simplifies the planning of a Gibson Assembly reaction, and automates the primer design. Select the DNA fragments that you wish to fuse, and SnapGene will design the primers. SnapGene is molecular biology software that allows users to plan, visualize, and document molecular biology procedures. Metscher BD (2009) Micro CT for comparative morphology: simple staining methods allow high-contrast 3D imaging of diverse non-mineralized animal tissues.SnapGene Frequently Asked Questions (FAQ) Overview Industrialized CRISPR Platforms for Accelerated Discovery to Clinic. T7 Endonuclease I assay – CRISPR fly design.īrinkman EK, Chen T, Amendola M, van Steensel B (2014) Easy quantitative assessment of genome editing by sequence trace decomposition. ![]() Nakayama T et al (2014) Cas9-based genome editing in xenopus tropicalis. Shen MW et al (2018) Predictable and precise template-free CRISPR editing of pathogenic variants. Naert T et al (2020) Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos. Moreno-Mateos MA et al (2015) CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo. ![]() Nieuwkoop PD, Faber J (1994) Normal table of Xenopus Laevis (Daudin): a systematical & chronological survey of the development from the fertilized egg till the end of metamorphosis. Hellsten U et al (2010) The genome of the western clawed frog xenopus tropicalis. Session AM et al (2016) Genome evolution in the allotetraploid frog Xenopus laevis. Macken WL et al (2021) Biallelic variants in COPB1 cause a novel, severe intellectual disability syndrome with cataracts and variable microcephaly. PLoS Genet 2:e91Ību-Daya A, Khokha MK, Zimmerman LB (2012) The hitchhiker’s guide to Xenopus genetics. Goda T et al (2006) Genetic screens for mutations affecting development of Xenopus tropicalis. Dev Biol 336:20–29Ību-Daya A et al (2011) The secreted integrin ligand nephronectin is necessary for forelimb formation in Xenopus tropicalis. Humana Press, pp 3–15Ību-Daya A, Sater AK, Wells DE, Mohun TJ, Zimmerman LB (2009) Absence of heartbeat in the Xenopus tropicalis mutation muzak is caused by a nonsense mutation in cardiac myosin myh6. In: Methods in molecular biology (Clifton, NJ), vol 917. Grainger RM (2012) Xenopus tropicalis as a model organism for genetics and genomics: past, present, and future. Sater AK, Moody SA (2017) Using Xenopus to understand human disease and developmental disorders. Hwang WY, Marquez J, Khokha MK (2019) Xenopus: driving the discovery of novel genes in patient disease and their underlying pathological mechanisms relevant for organogenesis. Mech Dev 146:1–9īlum M, Ott T (2018) Xenopus: an undervalued model organism to study and model human genetic disease. McQueen C, Pownall ME (2017) An analysis of MyoD-dependent transcription using CRISPR/Cas9 gene targeting in Xenopus tropicalis embryos. Hassnain Waqas SF et al (2017) Adipose tissue macrophages develop from bone marrow–independent progenitors in Xenopus laevis and mouse. Methods Mol Biol (Clifton, NJ) 127:111–123īhattacharya D, Marfo CA, Li D, Lane M, Khokha MK (2015) CRISPR/Cas9: an inexpensive, efficient loss of function tool to screen human disease genes in Xenopus. ![]() Guille M (1999) Microinjection into Xenopus oocytes and embryos. Sive HL, Grainger RM, Harland RM (2010) Microinjection of Xenopus embryos. Nakayama T et al (2013) Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis in Xenopus tropicalis. Jinek M et al (2012) A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Ishibashi S, Cliffe R, Amaya E (2012) Highly efficient bi-allelic mutation rates using TALENs in Xenopus tropicalis. Miller JC et al (2011) A TALE nuclease architecture for efficient genome editing. Nakajima K, Nakajima T, Takase M, Yaoita Y (2012) Generation of albino Xenopus tropicalis using zinc-finger nucleases. Genetics 188:773–782īibikova M, Golic M, Golic KG, Carroll D (2002) Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases. Science 343:84Ĭarroll D (2011) Genome engineering with zinc-finger nucleases. Shalem O et al (2014) Genome-scale CRISPR-Cas9 knockout screening in human cells. Drug Discov Today Technol 28:41–52ĭoudna JA, Charpentier E (2014) The new frontier of genome engineering with CRISPR-Cas9. Naert T, Vleminckx K (2018) CRISPR/Cas9 disease models in zebrafish and Xenopus: the genetic renaissance of fish and frogs.
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